The Colocalization Benchmark Source (CBS) project is a result of our over ten years research in quantification of colocalization of fluorescence markers. After publishing a number of papers, we realized that the biggest problem in this field of research is not how to obtain the quantitative colocalization data, but how to properly use the obtained results so that they are understandable and comparable between various analytical tools and labs. In our experience, the use of computer-simulated image sets with exactly known degrees of colocalization helps to solve this problem. Realizing that they can serve our fellow researchers as well and complying with their numerous requests to provide them, we are glad to share these image sets with the science community.

CBS was created by Vadim Zinchuk, M.D., Ph.D., Yong Wu, Ph.D., and Olga Grossenbacher-Zinchuk, M.D., Ph.D.

Selected references

Zinchuk V & Grossenbacher-Zinchuk O (2014) Quantitative colocalization analysis of fluorescence microscopy images. Curr Protoc Cell Biol 62:4.19.1‐4.19.14.

Zinchuk V et al (2013) Bridging the gap between qualitative and quantitative colocalization results in fluorescence microscopy studies. Sci Rep 3:1365 doi:10.1038/srep01365.

Zinchuk V et al (2011) Quantifying spatial correlations of fluorescent markers using enhanced background reduction with protein proximity index and correlation coefficient estimations. Nat Protoc 6:1554-1567.

Wu Y et al (2010) Quantitative determination of spatial protein-protein correlations in fluorescence confocal microscopy. Biophys J 98:493-504.

Related sources and databases

Database of Interacting Proteins

Human Protein Atlas

Protein Data Bank


Electron Microscopy Data Bank

Reference genome

Crystallographic database

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