The Colocalization Benchmark Source (CBS) project is the result of over ten years of our research into the quantification of colocalization of fluorescence markers. After publishing a number of papers, we realized that the biggest problem in this field is not how to obtain quantitative colocalization data, but how to properly use the results so that they are understandable and comparable across different analytical tools and laboratories. In our experience, using computer-simulated image sets with precisely known degrees of colocalization helps address this issue. Recognizing their value to fellow researchers and responding to numerous requests, we are pleased to share these image sets with the scientific community.
CBS was created by Vadym Zinchuk, M.D., Ph.D., Yong Wu, Ph.D., and Olga Grossenbacher-Zinchuk, M.D., Ph.D.
Zinchuk V & Grossenbacher-Zinchuk O (2014) Quantitative colocalization analysis of fluorescence microscopy images. Curr Protoc Cell Biol 62:4.19.1‐4.19.14.
Zinchuk V et al (2013) Bridging the gap between qualitative and quantitative colocalization results in fluorescence microscopy studies. Sci Rep 3:1365 doi:10.1038/srep01365.
Zinchuk V et al (2011) Quantifying spatial correlations of fluorescent markers using enhanced background reduction with protein proximity index and correlation coefficient estimations. Nat Protoc 6:1554-1567.
Wu Y et al (2010) Quantitative determination of spatial protein-protein correlations in fluorescence confocal microscopy. Biophys J 98:493-504.
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