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How to use

Step 1.

Download the appropriate set of benchmark images for the desired pair of channels.


Step 2.

Open this set of images in the quantitative colocalization analysis software you are using, and calculate the colocalization coefficients.


Step 3.

Compare the coefficient values obtained from your images with those from the benchmark images. Identify the benchmark image whose values most closely match yours, and use its colocalization score to describe your results.


If needed, you can split merged channels images into single channels images using CoLocalizer Pro software.

If you have any questions, check the FAQ before contacting us.


Image sets

Set 1:  Red-Green pair


Name


Colocalization


Type


Format


Size


Download


CBS001RGM


0%


Merged image


Lossless TIFF


1.7 MB


CBS002RGM


10%


Merged image


Lossless TIFF


1.7 MB


CBS003RGM


20%


Merged image


Lossless TIFF


1.7 MB


CBS004RGM


30%


Merged image


Lossless TIFF


1.7 MB


CBS005RGM


40%


Merged image


Lossless TIFF


1.7 MB


CBS006RGM


50%


Merged image


Lossless TIFF


1.7 MB


CBS007RGM


60%


Merged image


Lossless TIFF


1.7 MB


CBS008RGM


70%


Merged image


Lossless TIFF


1.7 MB


CBS009RGM


80%


Merged image


Lossless TIFF


1.7 MB


CBS010RGM


90%


Merged image


Lossless TIFF


1.7 MB


CBS001RGM-CBS010RGM


0-90%


Merged images


Lossless TIFFs


15.7 MB

Set 2:  Red-Blue pair


Name


Colocalization


Type


Format


Size


Download


CBS001RBM


0%


Merged image


Lossless TIFF


1.7 MB


CBS002RBM


10%


Merged image


Lossless TIFF


1.7 MB


CBS003RBM


20%


Merged image


Lossless TIFF


1.7 MB


CBS004RBM


30%


Merged image


Lossless TIFF


1.7 MB


CBS005RBM


40%


Merged image


Lossless TIFF


1.7 MB


CBS006RBM


50%


Merged image


Lossless TIFF


1.7 MB


CBS007RBM


60%


Merged image


Lossless TIFF


1.7 MB


CBS008RBM


70%


Merged image


Lossless TIFF


1.7 MB


CBS009RBM


80%


Merged image


Lossless TIFF


1.7 MB


CBS010RBM


90%


Merged image


Lossless TIFF


1.7 MB


CBS001RBM-CBS010RBM


0-90%


Merged images


Lossless TIFFs


15.7 MB

Set 3:  Green-Blue pair


Name


Colocalization


Type


Format


Size


Download


CBS001GBM


0%


Merged image


Lossless TIFF


1.7 MB


CBS002GBM


10%


Merged image


Lossless TIFF


1.7 MB


CBS003GBM


20%


Merged image


Lossless TIFF


1.7 MB


CBS004GBM


30%


Merged image


Lossless TIFF


1.7 MB


CBS005GBM


40%


Merged image


Lossless TIFF


1.7 MB


CBS006GBM


50%


Merged image


Lossless TIFF


1.7 MB


CBS007GBM


60%


Merged image


Lossless TIFF


1.7 MB


CBS008GBM


70%


Merged image


Lossless TIFF


1.7 MB


CBS009GBM


80%


Merged image


Lossless TIFF


1.7 MB


CBS010GBM


90%


Merged image


Lossless TIFF


1.7 MB


CBS001GBM-CBS010GBM


0-90%


Merged images


Lossless TIFFs


15.7 MB

Recommended citation


"Image set CBS001RGM-CBS010RGM from the Colocalization Benchmark Source (www.colocalization-benchmark.com) was used to validate colocalization"

License


The images are licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 Unported License.



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