Frequently Asked Questions

How close are the benchmark images to actual biomedical images?

The benchmark images are designed to model real-world scenarios in which colocalization occurs—specifically, when fluorescently labeled molecules A and B are located within a distance of one pixel. These signals are then convolved with the point spread function (PSF) of the fluorescence microscope used to detect them.

My images appear to have background noise, out-of-focus fluorescence, and bleedthrough. Can I still use them?

Before quantifying such images, we recommend correcting them (removing background noise) using available software tools. If you are unable to adequately correct for these issues, we do not recommend using CBS, as the images would not be suitable for reliable quantification—whether or not benchmark images are used.

Which colocalization coefficients do you recommend for comparing quantification results?

You may use any coefficients you prefer. The key is to ensure that you are comparing the same coefficients across your images and the benchmark images.

The pixel sizes of my images and the benchmark images do not match. Can I still use the CBS sets?

Yes, you can. The pixel size does not affect the characteristics relevant to colocalization quantification.

How should I cite the use of CBS in my publication?

The recommended citation format can be found on the Downloads page.